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Contents

Zeiss 200M microscope

Sign-up list

User / Date / Duration

e.g. Shay / whenever he wants / as long as it takes
e.g. Rotons / when Shay lets them / not long

Jeanne 06/12/11: 10.00-16.00 (37°C)
Raul 06/16/11: 16:00-21:00 (37°C)

Configuration

Objectives

Filter cubes

spare filter cubes:

http://www.semrock.com/Catalog/SetDetails.aspx?SetBasePartID=2
ex: 435/40 Dichroic: 510LP em: 605/15

Maintenance log

Solution: Adjust all settings so that shutter stays open and use shutter on DG4 light source (which is a lot faster anyways!)

Solution: as before, Christine changed all settings back to using DG4 shutter instead

PALM secret weapon

Configuration

Sign-up list

User / Date / Duration


Burak & Victor: 07/17/09: 10:00-18:00


Objectives

Position Objective
1 empty
2 10x A-Plan, 0.25 NA, Ph1
3 empty
4 40x LD Plan-NEOFLUAR, 0.6 NA, Korr, DIC(II)
5 63x, Plan-NEOFLUAR, 1.25 NA, oil immmersion, DIC(II)
6 100x FLUAR, 1.3 NA, oil immersion, DIC(III)

Usable with excision laser - apparently 100x FLUAR isn't high enough quality to be used for excision.

Filters

Excitation wheel:

Position Filters
1 blocked
2 BP390-420
3 D485/20X (55748)
4 D535/50X (60742) (splotchy on inner surface of filters?)
5 blocked
6 open
7,8 blocked


Emission cubes:

Position Filters
1 HR355+1064; HT395+VIS/45° (15AZ106812)
2 HR355+1064; HR390HT450+VIS (15AZ106815)
3 GFP (424931)
4 RFP: BP 565/30; FT 585; BP 620/60 (from other Zeiss; need to move excitation to Wheel?!?)

Maintenance log

Failed!!!! Jeff couldn't get PALM and AxioVision software to recognize the camera at the same time.




Nikon Ti microscope station #1 (aka "Mesut")

Sign-up list

User / Date / Duration


Dirk: 10/15/10: 100x TIRF objective will be on Nikon Ti #2; will not use Nikon Ti #1
Dirk: 10/19/10: 100x TIRF objective will be on Nikon Ti #2; will not use Nikon Ti #1
Dirk: 10/21/10: 100x TIRF objective will be on Nikon Ti #2; will not use Nikon Ti #1
Dirk and Burak: 11/5/10: 10:00 - 20:00
Dirk: 11/6/10: 100x TIRF objective will be on Nikon Ti #2; will not use Nikon Ti #1
Burak: 11/17/10: 10:00-14:00 - will use the 60x TIRF objective
Burak: 11/18/10: 10:00-17:00 - will use the 60x TIRF objective
Dirk/Nate: 11/23/10 10:00-14:00 (Using both TIs)
Burak: 11/23/10 14:00-19:00
Nate: 100x PA objective after Burak is done (overnight)

Burak: 11/24/10 10:00-18:00
Burak: 11/25/10 10:00-18:00 (60x TIRF objective)
Burak: 11/25/10 12:00-18:00 (60x TIRF objective)
nate/meriem: 11/30/10 10:00-O/N-- 100x PA Objective on TI-2
Burak: 12/02/10 12:00-18:00 (60x TIRF objective)
Burak: 12/03/10 12:00-18:00 (60x TIRF objective)
Dann: 12/07/10 15:00-17:00 (60x TIRF objective)
Burak: 12/08/10 13:00-18:00 (60x TIRF objective)
Dann: 12/09/10 15:00-18:00 (60x TIRF objective)
Burak: 12/10/10 14:00-18:00 (60x TIRF objective)
Burak: 12/14/10 17:00-20:00 (60x TIRF objective)
Burak: 01/25/11 16:00-22:00 (60x TIRF objective)
Meriem+Burak: 02/3/11 13:00-18:00 (100X PA objective)
Meriem+Burak: 02/8/11 13:00-18:00 (100X PA objective)
Meriem+Burak: 02/17/11 13:00-18:00 (100X PA objective)
Meriem+Burak: 06/28/11 13:00-18:00 (100X PA objective)


Configuration

General

Objectives

Cameras

Optional:

Filter cubes

spare filter cube:


filters for the lasers

dichroics sitting on the optical table, for diverting the beams:

ZT405RDC (chroma) - for 405 nm

ZT488RDC (chroma) - for 488 nm

ZT514RDC (chroma) - we have this for 514 nm but not the laser so this is NOT installed

LM01-552-25 (semrock) - for 532 nm

http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=859&CategoryID=83

dichroic: Di01-R532-25x36 (Semrock) http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=870&CategoryID=81
emission:FF01-607/70-25 (Semrock) http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=817&CategoryID=27

filters are sitting on a custom made brass filter cube

dichroic: Di01-R488 (Semrock) http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=775
emission 1: BLP01-488R-25 (Semrock) http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=94&CategoryID=78
emission 2: FF01-525/45 (Semrock) http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=787

Di01-R635-25x36

http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=777

Lasers

Maintenance log


To-do list:



Nikon Ti microscope station #2 (aka "Nuri")

What about Victoria -- crown princess of Sweden
Or Mameluk -- the famous turkish warriors (these guys were tough and could handle a large workload like the Nikon Ti ... hopefully)

Sign-up list

User / Date / Duration

Dirk: 5/02/10 8:00-8:01
Dann: 7/19/10 10:00-18:00 borrowing 100X objective
Dann: 7/27/10 19:00-22:00 borrowing 100X objective...Dirrrrrrrrrrkkkkkkkkk!!!!
Dirk: 8/16/10 12:00-20:0
Mason: 8/20/10 8:00-18:0 Change of hard drive
nate: 9/8/10: 15:00-O/N
Dirk: 9/9/10: 13:00-15:00
nate/Tom: 9/9/10: 15:00-O/N
Dirk: 9/10/10: 19:00-21:00
Nate/Tom: 9/13/10: 15:00-O/N
Dirk: 9/14/10: 12:00-20:00
Nate/Tom: 9/15/10: 12:00-O/N
Nate: 9/16/10: 16:00-O/N
Meriem/dirk: 9/17/10: 11:00-18:00
Dirk: 9/19/10: 14:00-20:00 change temperature to 30 C
Dirk: 9/20/10: 16:00-20:00
Meriem: 9/21/10: 11:00-17:00
Nate: 9/21/10: 17:00-O/N
Dirk: 9/23/10: 14:00-20:00 change temperature to 30 C, use 100x TIRF objective
Nate: 9/23/10: 20:00-O/N (37 C )
Meriem: 9/24/10: 11:00-17:00
Nate: 9/26/10: 14:00-20:00
Dirk: 9/27/10: 12:00-20:00
nate: 9/28/10: 17:00-O/N (Let me know if you want to use it in the morning!)
Dirk: 9/29/10: 13:00-20:00 change temperature to 30 C, use 100x TIRF objective
Dirk: 9/30/10: 12:00-20:00 change temperature to 37 C, use 100x TIRF objective
Meriem: 10/3/10: 10:00-14:00 (will probably finish earlier)
Nate: 10/5/10: 15:00-O/N Will set it to 37 around noon, if there are no objections
Dirk: 10/6/10: 12:00-20:00
Meriem: 10/7/10: 11:00-17:00
Dirk: 10/8/10: 12:00-20:00 change temperature to 37 C, use 100x TIRF objective
Dirk: 10/13/10: 12:00-20:00 change temperature to 37 C, use 100x TIRF objective
Nate: 10/13/10: 20:00-24:00
meriem:10/14/10: 11:00-15:00
Nate: 10/14/10: 17:00-O/N (will set to 37 C at ~13:00, if there are no objections)
Dirk: 10/15/10: 12:00-20:00 change temperature to 37 C, use 100x TIRF objective
Nate: 10/18/10: 15:00-20:00 (37 C at noon if possible)
Dirk: 10/19/10: 12:00-20:00 change temperature to 37 C, use 100x TIRF objective
Nate: 10/21/10: 20:00-O/N. Dirk: 10/21/10: 12:00-20:00 change temperature to 37 C, use 100x TIRF objective
Tom: 10/25/10: 3:00 - O/N (37 C at say 1 if possible)
Nate: 10/26/10: 17:00-O/N (37 a bit earlier, if no objections)
Tom: 10/27/10: 17:00-O/N (37 at say 2 if no one is using)
Meriem: 10/28/10: 11:00-20:00 (37 in the morning if possible)
Meriem: 10/31/10: 11:00-15:00
Nate: 10/31/10 : 16:00-O/N
Dirk: 11/01/10 : 16:00-20:00 (no heating, 100x PA objective)
Nate: 11/2/10: 17:00-O/N (37 C, 60X TIRF objective from TI-1 if possible)
Tom/Nate: 11/3/10: 17:00-O/N (37 C, 60x TIRF from TI-1)
meriem: 11/4/10 11:00-17:00(37C, 100x PA objective)
Dirk: 11/5/10: I will be using the 100x PA objective on Nikon Ti #1
Dirk: 11/6/10:15:00 - 20:00 (37 C, 100x TIRF objective)
meriem: 11/8/10 11:00-17:00(37C, 100x TIRF objective)
Tom/Nate: 11/10/10: 16:00-O/N (37 C, 60x TIRF from TI-1)
Nate: 11/11/10: 19:00-O/N (37 C)
Dirk: 11/12/10:12:00 - 20:00 (37 C, 100x TIRF objective)
Dirk: 11/15/10:12:00 - 20:00 (37 C, 100x TIRF objective)
Nate: 11/16/10: 17:00-O/N (37C, 100X PA objective)
Nate: 11/17/10: 17:00-O/N (37C, 100X PA objective)
Dirk: 11/18/10:12:00 - 20:00 (37 C, 100x TIRF objective)
Nate: 11/18/10: 20:00-O/N (37C, 100X PA objective)
Dirk: 11/19/10:12:00 - 20:00 (37 C, 100x TIRF objective)
Dirk/Nate: 11/23/10 10:00-14:00 (Using both TIs)
Nate: 11/23/10: 17:00-O/N 37 C, 100 PA objective
meriem: 11/24/10 10:00-18:00
Tom: 11/24/10: 18:00 - O/N 60X TIRF objective, 37 C
Dirk: 11/25/10: 12:00 - 20:00, 37 C, 100x TIRF
Dirk: 11/29/10: 17:00 - 20:00, RT, 100x TIRF
meriem: 11/30/10 11:00-17:00 100x PA 37C
Nate: 11/30/10: 17:00-O/N 100x PA 37 C
Nate: 12/1/10: 17:00 - O/N 100x PA 37 C
Dirk: 12/2/10: 12:00 - 20:00 100x TIRF
Nate: 12/3/10: 17:00-O/N 100x PA 37 C
Dirk: 12/5/10: 12:00 - 20:00 100x TIRF, 37 C
Dirk: 12/6/10: 12:00 - 16:00 100x TIRF, RT
Tom: 12/6/10: 16:00 - O/N 60X TIRF, 37 C
Nate: 12/7/10: 17:00 - O/N 100x PA 37 C
Nate/Jue: 12/8/10 10:00-14:00 100x PA RT
Dirk: 12/8/10: 17:00 - 19:00 100x PA, RT
Nate: 12/9/10: 17:00-O/N, 100x PA, 37 C
Dirk: 12/10/10: 12:00 - 20:00 100x TIRF, RT
Nate: 12/10/10: 20:00-O/N 100x PA 37 C
Tom: 12/11/10: 14:00 - O/N 60X TIRF, 37
Dirk: 12/12/10: 15:00 - 20:00 100x TIRF, RT
Dirk: 12/13/10: 15:00 - 20:00 100x TIRF, RT, if this is okay with Nate
Dirk: 12/14/10: 15:00 - 20:00 100x TIRF, RT, if this is okay with Nate
Nate: 12/14/10: 17:00-O/N 100x PA, 37 C
Dann: 12/15/10: 10:00 - 14:00 100x ?, RT
Tom: 12/15/10: 16:00-O/N 60X TIRF, 37 C
Nate: 12/16/10: 17:00-O/N 100x PA, 37 C
Dirk: 12/17/10: 12:00-22:00 100X TIRF, RT
Jue: 1/14/11: 14:00-18:00 100X PA, RT
Nate: 1/23/11: 16:00-O/N 100x PA, 37 C
Dirk: 1/24/11: 12:00-20:00 100X TIRF, RT
Nate: 1/24/11: 20:00-24:00 100X PA, RT
Nate: 1/25/11: 17:00-20:00 100x PA, RT
Nate: 1/27/11: 17:00-O/N 100x PA, RT
Nate: 1/29/11: 17:00-O/N 100x PA, RT
Dirk: 1/31/11: 16:00-18:00 if this is okay with Nate
Nate: 2/1/11: 17:00-O/N 100x PA, 37 C
Dann/Yoon: 4/19/11: 15:30-O/N 100x PA
Nate: 5/13/11-5/14/11: 17:00-O/N 100x PA
Nate: 5/16/11-5/17/11: 17:00-O/N 60x TIRF

Configuration

General

See "Certificate of Conformance" for details (upload file)

Measured LED light intensities out of the 20x objective with optical power meter (Thorlabs PM100D).
The LED intensities are set to 100% in the software. Field diaphragm is fully open.
The violet LED has to be measured at 400 nm because of technical limitations.

20100819 Date 2
1. Violet (400 nm) 14.8 mW Cell C
2. Blue (434 nm) 49.3 mW Cell C
3. Cyan (485 nm) 23.6 mW Cell C
4. Teal (513 nm) 10.5 mW Cell C
5. Green (549 nm) 47.8 mW Cell C
6. Yellow (586 nm) 21.7 mW Cell C
7. Red (650 nm) 7.75 mW Cell C


Flat-filed is pretty good with Lumencor but could be better


Serial ports:


Objectives

Cameras

Filter cubes

The filter cubes have no excitation filter because the excitation filter is in the Lumencor light engine.


The dichroics for GFP, YFP and mCherry could be more red-shifted.
All filter cubes, except the YFP and mCherry cubes, have only the dichroic and the emission filter in place. The excitation filters are in the LED. We still need excitation filters in the YFP and mCherry cubes to prevent excitation light leaking through the dichroic. Should be fixed soon.

Incubator

Turn incubator on ~3 hours before imaging to equilibrate the microscope to the new temperature.
The LED display of the incubator does not correspond to the correct temperature.
For 37 C, set the incubator to 32 (Nate: according to the digital thermometer) C.
For 30 C, set the incubator to 26.5 C.

Maintenance log



Work-around of known problems:



To-do list:




Nikon #1/2 Objectives

Objective/Nikon Microscope Sign Up